Journal: Molecular biomedicine

Article Title: Enteric glial cells aggravate the intestinal epithelial barrier damage by secreting S100β under high-altitude conditions.

doi: 10.1186/s43556-023-00143-1

Figure Lengend Snippet: Fig. 1 The levels of EGC biomarkers are significantly increased by HA conditions. a Serum concentrations of GFAP, S100β, GDNF, and NGF in control and HA group participants. b Relative mRNA expression per β-Actin of GFAP, S100β, GDNF, and NGF-β in the small intestines of control and HA group mice. c Immunohistochemical staining of the small intestine for GFAP and S100β. (n = 6–8 mice/group), Mean ± SD represent the findings. ****p < 0.0001, **p < 0.01, *p < 0.05. MOD—Mean Optical Density, IHC - immunohistochemistry

Article Snippet: To explore the role of S100β, MODE-K cells were treated with 5 μM recombinant mouse S100β (Solarbio, P00208) and subjected to hypoxia for 12 h, and control MODE-K cells were subjected to hypoxia alone.

Techniques: Control, Expressing, Immunohistochemical staining, Staining, Immunohistochemistry

Journal: Molecular biomedicine

Article Title: Enteric glial cells aggravate the intestinal epithelial barrier damage by secreting S100β under high-altitude conditions.

doi: 10.1186/s43556-023-00143-1

Figure Lengend Snippet: Fig. 5 S100β mediates the effect of EGCs to aggravate epithelial cell injury under hypoxic conditions. a Effect of exogenous S100β on MODE-K epithelial cell proliferation under hypoxic conditions. b Representative flow cytometric plots of MODE-K cell apoptosis. Annexin V and PI were used to label the cells before they were analyzed by flow cytometry. The figures show what proportion of the total occur in each quadrant. c Statistical analysis of the frequency percentage in late-phase apoptosis. d Relative mRNA expression per β-Actin of IL-6, -10, -1α, -1β, and TNF-α, claudin-1, occludin, and ZO-1. e Occludin and ZO-1 protein expression. Representation of findings and significance as previously stated

Article Snippet: To explore the role of S100β, MODE-K cells were treated with 5 μM recombinant mouse S100β (Solarbio, P00208) and subjected to hypoxia for 12 h, and control MODE-K cells were subjected to hypoxia alone.

Techniques: Flow Cytometry, Expressing

Journal: Journal of Korean Neurosurgical Society

Article Title: S100ß, Matrix Metalloproteinase-9, D-dimer, and Heat Shock Protein 70 Are Serologic Biomarkers of Acute Cerebral Infarction in a Mouse Model of Transient MCA Occlusion

doi: 10.3340/jkns.2017.0200

Figure Lengend Snippet: Plasma S100β concentration in all groups. The concentration of S100β increases with occlusion time (p=0.001; A) and correlates significantly with infarct volume (r=0.867, p <0.001; b) and neurological deficit (r=0.802, p <0.001; c). * p <0.05.

Article Snippet: The plasma concentrations of S100β, MMP-9, D-dimer, and HSP70 were calculated using mouse protein S100β (CSB-EL-0206643MO; CUSABIO, Wuhan, China), mouse total MMP-9 (MMPT90; R&D Systems, Minneapolis, MN, USA), mouse D-dimer (E03D0224; BlueGene, Shanghai, China), and mouse HSP70 (CSB-E08311m; CUSABIO) ELISA kits.

Techniques: Clinical Proteomics, Concentration Assay

Journal: Journal of Korean Neurosurgical Society

Article Title: S100ß, Matrix Metalloproteinase-9, D-dimer, and Heat Shock Protein 70 Are Serologic Biomarkers of Acute Cerebral Infarction in a Mouse Model of Transient MCA Occlusion

doi: 10.3340/jkns.2017.0200

Figure Lengend Snippet: Sensitivity and specificity of the biomarkers. MMP-9 and HSP70 both have an AUc of 1. S100β has an AUc of 0.98. The AUc for D-dimer is 0.58. MMP-9 : matrix metalloproteinase-9, HSP70 : heat shock protein 70, AUc : the area under the receiver operating characteristic curve.

Article Snippet: The plasma concentrations of S100β, MMP-9, D-dimer, and HSP70 were calculated using mouse protein S100β (CSB-EL-0206643MO; CUSABIO, Wuhan, China), mouse total MMP-9 (MMPT90; R&D Systems, Minneapolis, MN, USA), mouse D-dimer (E03D0224; BlueGene, Shanghai, China), and mouse HSP70 (CSB-E08311m; CUSABIO) ELISA kits.

Techniques: